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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
18/02/2021 |
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Data da última atualização: |
18/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA; VICENTE PALLÁS; JESÚS Á. SÁNCHEZ-NAVARRO. |
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Título: |
Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and capsid proteins. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Microorganisms, v.9,n.418, 2021. |
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DOI: |
https://doi.org/10.3390/microorganisms9020418 |
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Idioma: |
Inglês |
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Conteúdo: |
Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described theassociation of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Citrus; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01457naa a2200229 a 4500
001 2130068
005 2021-02-18
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/microorganisms9020418$2DOI
100 1 $aLEASTRO, M. O.
245 $aMembrane Association and Topology of Citrus Leprosis Virus C2 Movement and capsid proteins.$h[electronic resource]
260 $c2021
520 $aAlthough citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described theassociation of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed.
650 $aCitrus
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tMicroorganisms$gv.9,n.418, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 13 | |
| 7. |  | FAJARDO, T. V. M.; PEIRÓ, A.; SÁNCHEZ-NAVARRO, J. A.; PALLÁS, V. Role of the 5' untranslated region of the Alfalfa mosaic virus RNA 3 in cell-to-cell and long distance transport. Virología, Madrid, v. 16, n. 2, p. 143-144, 2013. Extraordinario. Edição dos Resumos do 12º Congreso Nacional de Virología, Burgos, Espanha, jun. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | SÁNCHEZ-NAVARRO, J. A.; FIORE, N.; FAJARDO, T. V. M.; PALLÁS, V. Simultaneous detection of the 13 viruses and 5 viroids affecting grapevine by molecular hybridization using a unique probe or 'polyprobe'. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 40-41.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Citrus LEPROSIS virus c codifica três proteínas com atividades supressoras de silenciamento gênico Citrus Leprosis Virus C encodes three proteins with gene silencing suppression activity. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 190. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | FIORE, N. UNES, M. Z.; ZAMORANO, A.; FAJARDO, T. V. M.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. A. Simultaneous detection of the main viruses and viroids affecting grapevine by molecular hybridization using a unique riboprobe or 'Polyprobe'. In: CONGRESS OF THE INTERNATIONAL COUNCIL FOR THE STUDY IF VIRUS AND VIRUS-LIKE DISEASES OF THE GRAPEVINE (ICVG), 17., 2012, Davis, CA. Proceedings... Davis: UCLA, Foundation Plant Services, 2012. p. 146-147.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | APARICIO, F.; FAJARDO, T. V. M.; HERRANZ, M. C.; PEIRÓ, A.; PALLÁS, V.; SANCHEZ-NAVARRO, J. A. The transport of plant viruses: a critical step in the viral cycle. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. p. 1312-1315. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Mesa redonda 10.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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